English Name: RecA protein

Level: br

Concentration: 2mg / ml

Unit definition: This product is sold according to the pure protein content. The amount of pure protein was measured under od280 (A280 value at protein concentration of 1 mg / ml: 0.516; optical path: 1 cm).

Thermal inactivation: 65 ℃, 20 min

Properties: powder or liquid. Product contains 1 ml of 10 × Reaction Buffer。 After many times of column purification, only a clear and single target band can be seen by SDS-PAGE gel detection; No E. coli DNA residue and no contamination of endonuclease and exonuclease were detected by PCR. E. Coli RecA protein is indispensable in gene recombination. It participates in DNA repair and UV induced mutation repair. RecA promotes the autolysis of LexA repressor, umud protein and lambda repressor. Cleavage of LexA can de repress more than 20 genes. In vitro studies have demonstrated that RecA promotes strand substitution between single stranded DNA fragments and homologous double stranded DNA fragments with the participation of ATP. The above reactions need three steps to complete: (I) RecA binds to single stranded DNA; (II) nucleoprotein filaments bind to double stranded DNA to search for homologous parts; (III) chain substitution. This product is obtained by expressing the RecA gene (E. coli) in E. coli and purifying and isolating it for many times.

Purpose: biochemical research. DNA structure was analyzed by electron microscopy; D-loop mutation; DNA library screening was performed with RecA protein coated probes; Cutting any predetermined site

Storage: - 20 ℃